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F-IRF7
(Plasmid #169777)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169777 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 169777-D50 50 μg of DNA in Tris buffer $495
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DNA 169777-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV-Tag2
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Total vector size (bp) 5812
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    interferon regulatory factor 7 (IRF7), transcript variant a
  • Alt name
    IRF7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1512
  • GenBank ID
    NM_001572
  • Entrez Gene
    IRF7 (a.k.a. IMD39, IRF-7, IRF-7H, IRF7A, IRF7B, IRF7C, IRF7H)
  • Tag / Fusion Protein
    • Flag tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T3P
  • 3′ sequencing primer T7P
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    F-IRF7 was a gift from Sung-Liang Yu (Addgene plasmid # 169777 ; http://n2t.net/addgene:169777 ; RRID:Addgene_169777)

  • For your References section:

    IKKepsilon isoform switching governs the immune response against EV71 infection. Chang YL, Liao YW, Chen MH, Chang SY, Huang YT, Ho BC, Yu SL. Commun Biol. 2021 Jun 2;4(1):663. doi: 10.1038/s42003-021-02187-x. 10.1038/s42003-021-02187-x PubMed 34079066
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