XE19 XIHbox 6
(Plasmid #16999)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)

Backbone

• Vector backbone: SP64T
• Backbone size w/o insert (bp): 3000
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: X1Hbox 6
• Alt name: hbox
• Alt name: hox
• Species: X. laevis (frog)
• Insert Size (bp): 700

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II, Bam H1 (destroyed during cloning)
• 3′ cloning site: Bgl II (unknown if destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: XIHbox 6 cDNA obtained by PCR amplification of neurula stage cDNA subcloned into SP64T.
Transcription: linearize with Bam H1 and transcribe with SP6.

Plasmid History:
Constructed by Jan Christian.