|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||170240||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9514
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1179
Entrez GeneMAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)
- Promoter EF1a
- Cloning method Gibson Cloning
- 5′ sequencing primer 5'-ttctcaagcctcagacagtgg-3' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode was a gift from Prashant Mali (Addgene plasmid # 170240 ; http://n2t.net/addgene:170240 ; RRID:Addgene_170240)
For your References section:Charting oncogenicity of genes and variants across lineages via multiplexed screens in teratomas. Parekh U, McDonald D, Dailamy A, Wu Y, Cordes T, Zhang K, Tipps A, Metallo C, Mali P. ISCIENCE (2021) 10.1016/j.isci.2021.103149