pEF1a-Usp9x_CD-mCherry
(Plasmid
#170380)
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PurposeVector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.
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Depositing Lab
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Depositing OrganizationUniversity of Toronto
Located in Canada -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170380 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 170380-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 170380-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEF1a-IRES-Neo (Plasmid #28019)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUbiquitin specific protease 9x catalytic domain
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Alt nameUsp9x
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SpeciesM. musculus (mouse)
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Insert Size (bp)2007
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MutationCatalytic domain
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GenBank IDNM_009481.2
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Entrez GeneUsp9x (a.k.a. 5730589N07Rik, AA407302, AA407699, AL022658, AL022749, Dffrx, FAF-X, Fafl)
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Tag
/ Fusion Protein
- mCherry-3xFlag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer taatacgactcactatagg
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe Usp9x catalytic domain was amplified from the full-length Usp9x ORF, obtained from DNASU.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 170380-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 170380-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF1a-Usp9x_CD-mCherry was a gift from Miguel Ramalho-Santos (Addgene plasmid # 170380 ; http://n2t.net/addgene:170380 ; RRID:Addgene_170380) -
For your References section:
The deubiquitinase Usp9x regulates PRC2-mediated chromatin reprogramming during mouse development. Macrae TA, Ramalho-Santos M. Nat Commun. 2021 Mar 25;12(1):1865. doi: 10.1038/s41467-021-21910-0. 10.1038/s41467-021-21910-0 PubMed 33767158
Map uploaded by the depositor.
