pEF1a-Usp9x_CD-mCherry
(Plasmid #170380)

• Purpose: Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.
• Depositing Lab: Miguel Ramalho-Santos
• Publication: Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi: 10.1038/s41467-021-21910-0.

Backbone

• Vector backbone: pEF1a-IRES-Neo (Plasmid #28019)
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin specific protease 9x catalytic domain
• Alt name: Usp9x
• Species: M. musculus (mouse)
• Insert Size (bp): 2007
• Mutation: Catalytic domain
• GenBank ID: NM_009481.2
• Entrez Gene: Usp9x (a.k.a. 5730589N07Rik, AA407302, AA407699, AL022658, AL022749, Dffrx, FAF-X, Fafl)
• Tag / Fusion Protein:
  • mCherry-3xFlag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: taatacgactcactatagg

Resource Information

• Supplemental Documents:
  • pef1a-usp9x_cd-mcherry-ires-neo.gb
• A portion of this plasmid was derived from a plasmid made by: The Usp9x catalytic domain was amplified from the full-length Usp9x ORF, obtained from DNASU.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry