Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


ZE10 sonic hedgehog pT7TS
(Plasmid #17059)

Full plasmid sequence is not available for this item.



This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17059 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone size w/o insert (bp) 3000

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    sonic hedgehog
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    shha (a.k.a. ShhNC, fc83d08, shh, syu, vhh-1, vhh1, wu:fc83d08)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site SpeI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Steve Ekker clone E, in vector pT7TS. Linearize with BamH1 and transcribe with T7 for sense RNA for injections.
Citation: Ekker et al. (1995), Current Biology 5:944-955.

Made by insertion of shh open-reading frame into the SpeI site of the pT7TS vector. The ORF was made by PCR using the following primers: shhA (5′-GGACTAG-TCACCATGCGGCTTTTGACGAGA-3′) and shhB (5′-AGCATACTAGTAGTCTCAGCTTGAGTTTACTGA-3′).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE10 sonic hedgehog pT7TS was a gift from Randall Moon (Addgene plasmid # 17059 ; ; RRID:Addgene_17059)
  • For your References section:

    Patterning activities of vertebrate hedgehog proteins in the developing eye and brain. Ekker SC, Ungar AR, Greenstein P, von Kessler DP, Porter JA, Moon RT, Beachy PA. Curr Biol. 1995 Aug 1. 5(8):944-55. 10.1016/S0960-9822(95)00185-0 PubMed 7583153