41 pBS119 d10L
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13065||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2950
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namefli genomic
SpeciesD. rerio (zebrafish)
Insert Size (bp)7017
Entrez Genefli1a (a.k.a. cb855, fli, fli-1, fli1, wu:fc45b11)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
An XbaI fragment of the zebrafish fli1 promoter that includes 1 kb upstream of exon 1 and 6 kb of intron 1 was subcloned into pBluescript from a PAC obtained by hybridization to the 5' end of the fli1 cDNA. An oligonucleotide linker containing NheI, SrfI, and AscI sites was cloned into an MluI site upstream of the fli1 start codon to give pBS119d10L.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:41 pBS119 d10L was a gift from Nathan Lawson (Addgene plasmid # 13065 ; http://n2t.net/addgene:13065 ; RRID:Addgene_13065)
For your References section:Notch signaling is required for arterial-venous differentiation during embryonic vascular development. Lawson ND, Scheer N, Pham VN, Kim CH, Chitnis AB, Campos-Ortega JA, Weinstein BM. Development. 2001 Oct . 128(19):3675-83. PubMed 11585794