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ZE29 Stbm-deltaN-6myc-pCS2+
(Plasmid #17071)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17071 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Stbm deltaN
  • Alt name
    strabismus
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    850
  • Mutation
    amino acids 240-526 only
  • Entrez Gene
    vangl2 (a.k.a. stbm, tri, vang)
  • Tag / Fusion Protein
    • Myc6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Zebrafish strabismus amino acids 240-526 with 6 c-myc epitopes at N-terminus; EcoRI-Xba1 insert.

For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase.

Plasmid History:
Species of Sequence Origin: Zebrafish.
Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE29 Stbm-deltaN-6myc-pCS2+ was a gift from Randall Moon (Addgene plasmid # 17071 ; http://n2t.net/addgene:17071 ; RRID:Addgene_17071)
  • For your References section:

    The planar cell-polarity gene stbm regulates cell behaviour and cell fate in vertebrate embryos. Park M, Moon RT. Nat Cell Biol. 2002 Jan;4(1):20-5. 10.1038/ncb716 PubMed 11780127