pcDNA3-ZnRed
(Plasmid
#170782)
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Purposecytosolic ZnRed zinc sensor with 166 nM and 20 uM Kd
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170782 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameZnRed
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SpeciesSynthetic
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Insert Size (bp)909
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MutationK255N
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GenBank IDKU867815
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
When selecting zinc biosensors for your particular application, please pay attention to the Kd. The Kd of the sensor has to match the concentration range of your particular experiments. Please note that the K255N mutation in ZnRed does not alter plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-ZnRed was a gift from Huiwang Ai (Addgene plasmid # 170782 ; http://n2t.net/addgene:170782 ; RRID:Addgene_170782) -
For your References section:
Single Fluorescent Protein-Based Indicators for Zinc Ion (Zn2+). Chen Z, Ai HW. Anal Chem. 2016 Sep 20;88(18):9029-36. doi: 10.1021/acs.analchem.6b01653. Epub 2016 Aug 29. 10.1021/acs.analchem.6b01653 PubMed 27539450
