Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

ZE38 pCS2P+ sp5l ORF
(Plasmid #17079)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17079 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2P+ (modified pCS2+)
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sp5l ORF
  • Alt name
    sp5-like
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1100
  • Entrez Gene
    sp5l (a.k.a. Spr2, YF-335, cb577, fc23b01, s, sb:cb57, sb:cb577, wu:fc23b01)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: zebrafish sp5-like (sp5l) zinc-finger transcription factor of the SP5 family (cDNA clone 27i2 from the Wnt8 upregulated microarray screen) ORF only in pCS2P+. PCR amplified using primers 96 &97 from 27-i2 (pSPORT) clone and cloned EcoRI/XbaI into pCS2P+. Injection of high doses (>260 ng/�l corresponding to 200ng/�l #53.GFP) results in loss of eyes.

Species of Sequence Origin: zebrafish.

Notes for Use: For functional synthetic RNA, linearize with NotI, SP6 (1385bp RNA). Select clones with amp.
Reference: Development, Current Biology papers from the lab (2005).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE38 pCS2P+ sp5l ORF was a gift from Randall Moon (Addgene plasmid # 17079 ; http://n2t.net/addgene:17079 ; RRID:Addgene_17079)