ZE38 pCS2P+ sp5l ORF
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17079||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCS2P+ (modified pCS2+)
- Backbone size w/o insert (bp) 4100
Vector typeZebrafish Expression
Growth in Bacteria
Gene/Insert namesp5l ORF
SpeciesD. rerio (zebrafish)
Insert Size (bp)1100
Entrez Genesp5l (a.k.a. Spr2, YF-335, cb577, fc23b01, s, sb:cb57, sb:cb577, wu:fc23b01)
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Description: zebrafish sp5-like (sp5l) zinc-finger transcription factor of the SP5 family (cDNA clone 27i2 from the Wnt8 upregulated microarray screen) ORF only in pCS2P+. PCR amplified using primers 96 &97 from 27-i2 (pSPORT) clone and cloned EcoRI/XbaI into pCS2P+. Injection of high doses (>260 ng/�l corresponding to 200ng/�l #53.GFP) results in loss of eyes.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA, linearize with NotI, SP6 (1385bp RNA). Select clones with amp.
Reference: Development, Current Biology papers from the lab (2005).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE38 pCS2P+ sp5l ORF was a gift from Randall Moon (Addgene plasmid # 17079 ; http://n2t.net/addgene:17079 ; RRID:Addgene_17079)