ZE46 DeltaPGB Dvl2 pCS2P+
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17087||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeZebrafish Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt nameDishevelled 2
SpeciesD. rerio (zebrafish)
MutationDeltaPGB (deletes amino acids 231-252)
Entrez Genedvl2 (a.k.a. sb:eu471, wu:fc05d12, wu:fo71e09, wu:fp54a02, zgc:55372)
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Dishevelled 2 deleted in amino acids 231-252, cloned into Cla/EcoR1 sites.
Notes for Use: For functional synthetic RNA cut ASP718, transcribe SP6.
Select clones with amp.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE46 DeltaPGB Dvl2 pCS2P+ was a gift from Randall Moon (Addgene plasmid # 17087 ; http://n2t.net/addgene:17087 ; RRID:Addgene_17087)