XE123 XDsh delta PDZ-GFP- CS2+
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16786||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeXenopus Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesX. laevis (frog)
Mutationcontains Dsh amino acids 1-267 and 381-736 (deleting 268-380)
Entrez Genedvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996)
was engineered to now contain Dsh amino acids 1-267 and 381-736 (deleting 268-380).
Linearize with Not1 and transcribe with SP6.
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE123 XDsh delta PDZ-GFP- CS2+ was a gift from Randall Moon (Addgene plasmid # 16786 ; http://n2t.net/addgene:16786 ; RRID:Addgene_16786)