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Addgene

XE125 XDsh delta COOH-GFP-CS2+
(Plasmid #16784)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16784 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xdsh-delta-COOH
  • Alt name
    dsh
  • Species
    X. laevis (frog)
  • Mutation
    contains Dsh amino acids 1-588 (deleting 599-736)
  • Entrez Gene
    dvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
  • Tag / Fusion Protein
    • GFP

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996) was engineered to now contain Dsh amino acids 1-588 (deleting 599-736).

Linearize with Not1 and transcribe with SP6.

Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE125 XDsh delta COOH-GFP-CS2+ was a gift from Randall Moon (Addgene plasmid # 16784 ; http://n2t.net/addgene:16784 ; RRID:Addgene_16784)
  • For your References section:

    Establishment of the dorsal-ventral axis in Xenopus embryos coincides with the dorsal enrichment of dishevelled that is dependent on cortical rotation. Miller JR, Rowning BA, Larabell CA, Yang-Snyder JA, Bates RL, Moon RT. J Cell Biol. 1999 Jul 26;146(2):427-37. doi: 10.1083/jcb.146.2.427. 10.1083/jcb.146.2.427 PubMed 10427095