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XE124 XDsh delta DEP-GFP-CS2+
(Plasmid #16785)


Item Catalog # Description Quantity Price (USD)
Plasmid 16785 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    X. laevis (frog)
  • Mutation
    contains Dsh amino acids 1-433 and 503-736 (deleting 434-502)
  • Entrez Gene
    dvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
  • Tag / Fusion Protein
    • GFP

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996) was engineered to now contain Dsh amino acids 1-433 and 503-736 (deleting 434-502).

Linearize with Not1 and transcribe with SP6.

Notes for Use:
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.

Please note that this gene matches NM_001090627.1, NOT BC090218.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE124 XDsh delta DEP-GFP-CS2+ was a gift from Randall Moon (Addgene plasmid # 16785 ; ; RRID:Addgene_16785)
  • For your References section:

    Establishment of the dorsal-ventral axis in Xenopus embryos coincides with the dorsal enrichment of dishevelled that is dependent on cortical rotation. Miller JR, Rowning BA, Larabell CA, Yang-Snyder JA, Bates RL, Moon RT. J Cell Biol. 1999 Jul 26;146(2):427-37. doi: 10.1083/jcb.146.2.427. 10.1083/jcb.146.2.427 PubMed 10427095