XE124 XDsh delta DEP-GFP-CS2+
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16785||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeXenopus Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesX. laevis (frog)
Mutationcontains Dsh amino acids 1-433 and 503-736 (deleting 434-502)
Entrez Genedvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996) was engineered to now contain Dsh amino acids 1-433 and 503-736 (deleting 434-502).
Linearize with Not1 and transcribe with SP6.
Notes for Use:
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
Please note that this gene matches NM_001090627.1, NOT BC090218.1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE124 XDsh delta DEP-GFP-CS2+ was a gift from Randall Moon (Addgene plasmid # 16785 ; http://n2t.net/addgene:16785 ; RRID:Addgene_16785)