Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

XE124 XDsh delta DEP-GFP-CS2+
(Plasmid #16785)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16785 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xdsh-delta-DEP
  • Alt name
    dsh
  • Species
    X. laevis (frog)
  • Mutation
    contains Dsh amino acids 1-433 and 503-736 (deleting 434-502)
  • Entrez Gene
    dvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
  • Tag / Fusion Protein
    • GFP

Cloning Information

Resource Information

Depositor Comments

GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996) was engineered to now contain Dsh amino acids 1-433 and 503-736 (deleting 434-502).

Linearize with Not1 and transcribe with SP6.

Notes for Use:
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.

Please note that this gene matches NM_001090627.1, NOT BC090218.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE124 XDsh delta DEP-GFP-CS2+ was a gift from Randall Moon (Addgene plasmid # 16785 ; http://n2t.net/addgene:16785 ; RRID:Addgene_16785)