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Addgene

XE43 Xdsh-GFP
(Plasmid #16788)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16788 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xdsh-GFP
  • Alt name
    Xdsh
  • Species
    X. laevis (frog)
  • Entrez Gene
    dvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Transcription: linearize with Not I and transcribe with SP6.
Plasmid History:
Constructed by Randall T. Moon.
Notes for Use:
Xdsh of S. Sokol with GFP of Heim et al., Nature 1995, cloned at the carboxy terminal end, followed by a translation stop codon.
Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996.

Addgene QC found 2 point mutations, T511A and T717S, that do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE43 Xdsh-GFP was a gift from Randall Moon (Addgene plasmid # 16788 ; http://n2t.net/addgene:16788 ; RRID:Addgene_16788)
  • For your References section:

    A frizzled homolog functions in a vertebrate Wnt signaling pathway. Yang-Snyder J, Miller JR, Brown JD, Lai CJ, Moon RT. Curr Biol. 1996 Oct 1. 6(10):1302-6. 10.1016/S0960-9822(02)70716-1 PubMed 8939578