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XE122 XDsh delta DIX-GFP-CS2+
(Plasmid #16787)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16787 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xdsh-delta DIX
  • Alt name
    dsh
  • Species
    X. laevis (frog)
  • Mutation
    deletion of aa 1-159
  • Entrez Gene
    dvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
  • Tag / Fusion Protein
    • gfp

Cloning Information

Resource Information

Depositor Comments

XDsh-GFP is from J. Yang-Snyder et al. (Curr. Biol., 1996).
A deletion of amino acids 1-159 was conducted and a new start met was introduced.

Linearize with Not1 and transcribe with SP6.

Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE122 XDsh delta DIX-GFP-CS2+ was a gift from Randall Moon (Addgene plasmid # 16787 ; http://n2t.net/addgene:16787 ; RRID:Addgene_16787)