XE122 XDsh delta DIX-GFP-CS2+
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16787||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeXenopus Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameXdsh-delta DIX
SpeciesX. laevis (frog)
Mutationdeletion of aa 1-159
Entrez Genedvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
XDsh-GFP is from J. Yang-Snyder et al. (Curr. Biol., 1996).
A deletion of amino acids 1-159 was conducted and a new start met was introduced.
Linearize with Not1 and transcribe with SP6.
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE122 XDsh delta DIX-GFP-CS2+ was a gift from Randall Moon (Addgene plasmid # 16787 ; http://n2t.net/addgene:16787 ; RRID:Addgene_16787)