AKAP1-EGFP-uTEV1
(Plasmid
#171005)
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PurposeHiLITR protease with AKAP1 N-terminal tmd targeting information (Mitochondria)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171005 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCW-Cas9
- Backbone size w/o insert (bp) 8000
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Vector typeMammalian Expression, Lentiviral, Synthetic Biology
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAKAP1(tmd)-EGFP-uTEV1
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SpeciesSynthetic
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Insert Size (bp)1800
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Entrez GeneAKAP1 (a.k.a. AKAP, AKAP121, AKAP149, AKAP84, D-AKAP1, PPP1R43, PRKA1, SAKAP84, TDRD17)
- Promoter pTRE-tight (TetOn)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AKAP1-EGFP-uTEV1 was a gift from Alice Ting (Addgene plasmid # 171005 ; http://n2t.net/addgene:171005 ; RRID:Addgene_171005) -
For your References section:
An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens. Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, Weissman JS, Carr SA, Bassik MC, Ting AY. Elife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142. 10.7554/eLife.69142 PubMed 34414886
