An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens.
Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, Weissman JS, Carr SA, Bassik MC, Ting AY
Elife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142. PubMed Article
Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, Weissman JS, Carr SA, Bassik MC, Ting AY
Elife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170994 | UAS:mCherry | UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable. |
| 170995 | AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5 | HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria) |
| 170996 | TMED3-hLOV-TEVcs-GAL4bd-VP64-V5 | HiLITR transcription factor with full-length TMED3 targeting sequence (ER/Golgi) |
| 170997 | PMP34-hLOV-TEVcs-GAL4bd-VP64-V5 | HiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome) |
| 170998 | EGFP-uTEV1-MAVS | HiLITR protease with MAVS c-terminal tmd targeting information (Mitochondria) |
| 170999 | EGFP-uTEV1-MAVS-mTA | HiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion) |
| 171000 | EGFP-uTEV1-MAVS-mTA2 | HiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion) |
| 171001 | EGFP-uTEV1-MAVS-mTA3 | HiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion) |
| 171002 | EGFP-uTEV1-MAVS-mTA4 | HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion) |
| 171003 | EGFP-uTEV1-MAVS-mTA5 | HiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion) |
| 171004 | EGFP-uTEV1-MAVS-mTA6 | HiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria) |
| 171005 | AKAP1-EGFP-uTEV1 | HiLITR protease with AKAP1 N-terminal tmd targeting information (Mitochondria) |
| 171006 | EGFP-uTEV1-NES | HiLITR protease with C-terminal NNES (Cytoplasmic) |
| 171007 | EGFP-uTEV1-CB5 | HiLITR protease with CB5 C-terminal tmd targeting information (ER) |
| 171008 | EGFP-uTEV1-ACBD5 | HiLITR protease with ACBD5 C-terminal targeting information (peroxisome) |
| 171009 | EGFP-uTEV1-SQStmd | HiLITR protease with SQS C-terminal targeting information (ER) |
| 171010 | EGFP-uTEV1-SQSfl | HiLITR protease with full-length SQS targeting information (ER) |
| 171011 | pMCB619 | CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites). |
| 171012 | pLX208-SAE1 | Ectopic expression of SAE1 |
| 171013 | pLX208-nfBFP | Ectopic expression of nonfluorescent BFP |