pCI-Flag-NEDD4L∆WW1,2,4
(Plasmid
#171137)
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Purposeexpresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171137 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCI-FLAG
- Backbone size w/o insert (bp) 5842
- Total vector size (bp) 8047
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNEDD4L
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SpeciesH. sapiens (human)
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Insert Size (bp)2565
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Mutationchanged tryptophan 221 to alanine, tryptophan 453 to alanine, tryptophan 576 to alanine
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TAATACGACTCACTATAGG
- 3′ sequencing primer GCATTAACCCTCACTAAAG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-Flag-NEDD4L∆WW1,2,4 was a gift from Wesley Sundquist (Addgene plasmid # 171137 ; http://n2t.net/addgene:171137 ; RRID:Addgene_171137)