pG-HIV-LRT
(Plasmid #104592)

• Purpose: Standard for qPCR
• Depositing Lab: Kristine Yoder
• Publication: Yoder et al J Virol Methods. 2008 Nov;153(2):253-6. doi: 10.1016/j.jviromet.2008.07.032. Epub 2008 Sep 17.

Backbone

• Vector backbone: pGemT
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3001
• Vector type: cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIV-1 late reverse transcripts
• Species: HIV-1 virus

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7-F TAATACGACTCACTATAGGG and SP6-F ATTTAGGTGACACTATAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid may be used as a qPCR standard for the measure of HIV-1 virus. It encodes a short fragment of the HIV-1 genome corresponding to 557-699 bp in the HXB2 reference strain sequence. This sequence corresponds to 674-816 bp in reverse in the Addgene sequence file. It may be amplified with primers MH531 (5' TGTGTGCCCGTCTGTTGTGT), MH532 (5' GAGTCCTGCGTCGAGAGAGC), and Taqman probe LRT-P (5' CAGTGGCGCCCGAACAGGGA)