pENTR221-BNT162b2ΔUTR
(Plasmid
#171214)
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PurposeGateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171214 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4762
- Total vector size (bp) 8596
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Vector typeGateway cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBNT162b2ΔUTR
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SpeciesH. sapiens (human)
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Insert Size (bp)3834
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MutationKozak sequence changed from CCCGCCACC to GGCTGCACC.
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.medrxiv.org/content/10.1101/2021.04.27.21256151v1 for medRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR221-BNT162b2ΔUTR was a gift from Liang Wei Wang (Addgene plasmid # 171214 ; http://n2t.net/addgene:171214 ; RRID:Addgene_171214) -
For your References section:
BNT162b2 vaccination induces SARS-CoV-2 specific antibody secretion into human milk with minimal transfer of vaccine mRNA. Low JM, Gu Y, Ng MSF, Amin Z, Lee LY, Ng YPM, Shunmuganathan B, Niu Y, Gupta R, Tambyah PA, MacAry PA, Wang LW, Zhong Y. medRxiv 2021.04.27.21256151 10.1101/2021.04.27.21256151
