LV-AR-KKKAAA
(Plasmid #171219)

• Purpose: Lenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.
• Depositing Lab: Steven Balk
• Publication: Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi: 10.1038/s41467-021-27077-y.

Backbone

• Vector backbone: Lenti-viral-Hygro
• Backbone size w/o insert (bp): 10000
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AR-KKKAAA
• Alt name: AR-K630A-K632A-K633A
• Alt name: AR KLKK mutant
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Mutation: AR-K630A-K632A-K633A
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: EF-1a
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mutations occur on the nuclear localization signal (NLS) KLKK motif at the boundary between AR DBD and hinge domain. The mutant is defective in both nuclear import and transaction, and completely loses importin-alpha binding.

Human androgen receptor reference: https://pubmed.ncbi.nlm.nih.gov/18319300/

Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.