LV-AR
(Plasmid #213819)

• Purpose: Lenti-viral expression of human androgen receptor wild-type (WT).
• Depositing Lab: Steven Balk
• Publication: Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi: 10.1038/s41467-021-27077-y.

Backbone

• Vector backbone: Lenti-viral-Hygro
• Backbone size w/o insert (bp): 10000
• Total vector size (bp): 13110
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AR (human androgen receptor)
• Alt name: NR3C4
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Mutation: No, it is AR wild-type.
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: EF-1a
• Tag / Fusion Protein:
  • Flag (N terminal on backbone) (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EF1a-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The current LV-AR vector expresses a wild-type (WT) AR minigene. It shares the same backbone and plasmid map as the following Addgene deposits that express different AR mutants (
LV-AR-R599A-N600A, Plasmid#171221; LV-AR-C619Y, Plasmid#171222;
LV-AR-C563A-C596A, Plasmid#171220; LV-AR-KKKAAA, Plasmid#171219; LV-AR-AAFF, Plasmid#171218).
https://www.addgene.org/search/catalog/plasmids/?q=LV-AR
Notes:
1, human AR has different versions that are based on different races.
2, the organization of the AR expression cassette is as below: N-terminal of the AR gene is 3x-Flag tag and its C-terminal is in fusion with T2A-Hygromycin B.