|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||171222||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 10000
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)3000
Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter EF-1a
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Unknown
Terms and Licenses
- Not Available to Industry
The mutation occurs at the end of androgen receptor zinc finger 2 and this AR mutant is defective in transactivation but not nuclear import.
Human androgen receptor reference: https://pubmed.ncbi.nlm.nih.gov/18319300/
Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LV-AR-C619Y was a gift from Steven Balk (Addgene plasmid # 171222 ; http://n2t.net/addgene:171222 ; RRID:Addgene_171222)