|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||171251||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Gly mutant
SpeciesH. sapiens (human)
Insert Size (bp)3000
Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter CMV
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV_fwd_primer (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Flag-tagged human AR Ser-Pro mutant that binds to bicalutamide, and the exact amino position and composition may slightly vary based on human AR versions.Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Flag-M4-AR-W741C-S81A-P82G was a gift from Steven Balk (Addgene plasmid # 171251 ; http://n2t.net/addgene:171251 ; RRID:Addgene_171251)