pGEMHE-mScarlet-MAP4-MTBD
(Plasmid #171488)

• Purpose: T7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNA
• Depositing Lab: Melina Schuh
• Publication: Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00492-X. doi: 10.1016/j.cell.2021.04.013.

Backbone

• Vector backbone: pGEHME
• Vector type: pGEHME

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MAP4-MTBD
• Species: M. musculus (mouse)
• Mutation: Aminoacids 658-1126
• Entrez Gene: Map4 (a.k.a. AA407148, MAP-4, Mtap-4, Mtap4)
• Promoter: T7
• Tag / Fusion Protein:
  • mScarlet (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13: GTAAAACGACGGCCAGT
• 3′ sequencing primer: GGCTACATTTTGGGGGACAACATTTTGTAAAGT

Resource Information

• Supplemental Documents:
  • pGEMHE-mScarlet-MAP4-MTBD.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the Microtubule binding domain of MAP4.

5' cloning site: XhoI (unknown if destroyed), 3' cloning site: XbaI (unknown if destroyed).