pJC5-4-insulator-BREreporter-insulator
(Plasmid
#171499)
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Purposeinjections to make Bmp reporter mouse transgenic line
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Depositing Lab
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Depositing OrganizationFrancis Crick Institute
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171499 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 171499-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 171499-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGEM47
- Backbone size w/o insert (bp) 2750
- Total vector size (bp) 3950
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameInsulator BRE-MLP:H2B-Turqoise
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SpeciesM. musculus (mouse), Synthetic
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Insert Size (bp)6890
- Promoter BRE-MLP
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer cactcattaggcaccccag
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene QC NGS analysis was unable to generate a full plasmid sequence. The depositing laboratory has indicated that these sequence discrepancies should not be of any consequence to function. Please visit https://www.biorxiv.org/content/10.1101/2020.07.12.198994v2 for bioRxiv preprint.
DNA (Catalog # 171499-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 171499-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJC5-4-insulator-BREreporter-insulator was a gift from James Briscoe (Addgene plasmid # 171499 ; http://n2t.net/addgene:171499 ; RRID:Addgene_171499) -
For your References section:
Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak. Ivanovitch K, Soro-Barrio P, Chakravarty P, Jones RA, Bell DM, Mousavy Gharavy SN, Stamataki D, Delile J, Smith JC, Briscoe J. PLoS Biol. 2021 May 17;19(5):e3001200. doi: 10.1371/journal.pbio.3001200. eCollection 2021 May. 10.1371/journal.pbio.3001200 PubMed 33999917