|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17154||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Gene/Insert nametwhh promoter
Alt nametiggy-winkle hedgehog
SpeciesD. rerio (zebrafish)
Insert Size (bp)5200
Entrez Geneshhb (a.k.a. twh, twhh)
/ Fusion Protein
- b-globin intron, GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
Insert: twhh promoter (5.2 Kb), rabbit Beta-globin intron 2 (IVS2, 640 bp), GFP (65C, 1Kb)
Notes for Use:
Gives both notochord and floor-plate specific expression of GFP, owing to having the intron present.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M17 ptwhh-IGFP65C was a gift from Randall Moon (Addgene plasmid # 17154 ; http://n2t.net/addgene:17154 ; RRID:Addgene_17154)