|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17156||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Growth in Bacteria
SpeciesG. gallus (chicken)
Entrez GeneGDF2 (a.k.a. DSL1)
/ Fusion Proteins
- twhh promoter (D rerio) (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
To place dsl-1myc after the twhh promoter, the DNA insert of dsl-1myc was first subcloned into the EcoRI site of expression vector pCS2+, which has a cytomegalovirus promoter and a polyadenylation site. The resulting plasmids were named pCS2+-dsl-1myc. To link dsl-1myc to the twhh promoter, the dsl-1myc insert was released from pCS2+-dsl-1myc by BamHI and XhoI digestion and then subcloned into pCS-twhh-beta -gal-vec BamHI and XhoI sites by replacing the beta -gal sequence. The final construct, pCS-twhh-dsl-1myc, contains the 5.2-kb twhh promoter and dsl-1myc.
Notes for Use:
Target dorsalin-1 expression in zebrafish notochord.
Citation: Du et al., 1997, J. Cell Biology.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M19 pCS-twhh-dsl-1myc was a gift from Randall Moon (Addgene plasmid # 17156 ; http://n2t.net/addgene:17156 ; RRID:Addgene_17156)
Map uploaded by the depositor.