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M19 pCS-twhh-dsl-1myc
(Plasmid #17156)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17156 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dorsalin-1
  • Alt name
    dsl-1
  • Species
    G. gallus (chicken)
  • Entrez Gene
    GDF2 (a.k.a. DSL1)
  • Tags / Fusion Proteins
    • twhh promoter (D rerio) (N terminal on insert)
    • myc-tag

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Plasmid History:

To place dsl-1myc after the twhh promoter, the DNA insert of dsl-1myc was first subcloned into the EcoRI site of expression vector pCS2+, which has a cytomegalovirus promoter and a polyadenylation site. The resulting plasmids were named pCS2+-dsl-1myc. To link dsl-1myc to the twhh promoter, the dsl-1myc insert was released from pCS2+-dsl-1myc by BamHI and XhoI digestion and then subcloned into pCS-twhh-beta -gal-vec BamHI and XhoI sites by replacing the beta -gal sequence. The final construct, pCS-twhh-dsl-1myc, contains the 5.2-kb twhh promoter and dsl-1myc.

Notes for Use:
Target dorsalin-1 expression in zebrafish notochord.

Citation: Du et al., 1997, J. Cell Biology.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M19 pCS-twhh-dsl-1myc was a gift from Randall Moon (Addgene plasmid # 17156 ; http://n2t.net/addgene:17156 ; RRID:Addgene_17156)