E3_pENTR_L3-L2_NG_G1397-DddtoxA-N
(Plasmid
#171735)
-
PurposeptpTALECD vector assembly, Entry clone 1 with attL2 and L3
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171735 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonePINII
-
Vector typeMultisite Gateway entry vector
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameC- and N termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
-
SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Multisite Gateway entry vector to express one hand of pTALECD-UGI. Use th Platinum Gate TALEN Kit (Kit # 1000000054 ) for assembling of TALE repeats, in which use this plasmid in the last step.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
E3_pENTR_L3-L2_NG_G1397-DddtoxA-N was a gift from Shin-ichi Arimura (Addgene plasmid # 171735 ; http://n2t.net/addgene:171735 ; RRID:Addgene_171735) -
For your References section:
Targeted base editing in the plastid genome of Arabidopsis thaliana. Nakazato I, Okuno M, Yamamoto H, Tamura Y, Itoh T, Shikanai T, Takanashi H, Tsutsumi N, Arimura SI. Nat Plants. 2021 Jul;7(7):906-913. doi: 10.1038/s41477-021-00954-6. Epub 2021 Jul 1. 10.1038/s41477-021-00954-6 PubMed 34211131
