M24 Nac>Nac
(Plasmid
#17174)
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Depositing Lab
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Depositing OrganizationUniversity of Washington
Located in United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 17174-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 17174-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonen/a
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenacre promoter driving nacre cDNA
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Alt namenacre
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SpeciesD. rerio (zebrafish)
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GenBank IDAF211890
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Entrez Genemitfa (a.k.a. nacre, z3A.1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note for Use:
Description: Zebrafish nacre promoter driving nacre cDNA.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
DNA (Catalog # 17174-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 17174-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M24 Nac>Nac was a gift from Randall Moon (Addgene plasmid # 17174 ; http://n2t.net/addgene:17174 ; RRID:Addgene_17174) -
For your References section:
Direct regulation of nacre, a zebrafish MITF homolog required for pigment cell formation, by the Wnt pathway. Dorsky RI, Raible DW, Moon RT. Genes Dev. 2000 Jan 15;14(2):158-62. PubMed 10652270
Map uploaded by the depositor.
