pBI/Rabaptin-5
(Plasmid
#171851)
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Purposeinducible expression of Rabaptin-5 in mammalian cells via co-transfection with pTet-Off.
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Depositing Lab
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Depositing OrganizationUniversity of Oklahoma Health Sciences Center
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171851 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 171851-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 171851-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBI
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Backbone manufacturerTakara Biotech/Clontech
- Backbone size w/o insert (bp) 4358
- Total vector size (bp) 6947
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRabaptin-5 (human)
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SpeciesH. sapiens (human)
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Insert Size (bp)2589
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GenBank IDNP_004694.2
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Entrez GeneRABEP1 (a.k.a. RAB5EP, RABPT5)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Marino Zerial's group at MPI-CBG in Germany
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 171851-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 171851-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBI/Rabaptin-5 was a gift from Guangpu Li (Addgene plasmid # 171851 ; http://n2t.net/addgene:171851 ; RRID:Addgene_171851) -
For your References section:
Delayed onset of positive feedback activation of Rab5 by Rabex-5 and Rabaptin-5 in endocytosis. Zhu H, Qian H, Li G. PLoS One. 2010 Feb 16;5(2):e9226. doi: 10.1371/journal.pone.0009226. 10.1371/journal.pone.0009226 PubMed 20169068
