pMito-MtrxRFP2
(Plasmid #172324)

• Purpose: fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
• Depositing Lab: Huiwang Ai
• Publication: Pang et al Biochemistry. 2022 Mar 1;61(5):377-384. doi: 10.1021/acs.biochem.1c00634. Epub 2022 Feb 8.

Backbone

• Vector backbone: pCS2+
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
• Alt name: MtrxRFP2 with mitochondrial localization sequence
• Species: Synthetic
• Promoter: CMV, SP6
• Tags / Fusion Proteins:
  • His-tag (N terminal on insert)
  • Mitochondria localization sequence (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: SP6-Forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We recommend the use of pMito-MtrxRFP2 over pMito-TrxRFP1 (Plasmid #98997) for monitoring the redox of mitochondrial thioredoxin