pBabe 3xFLAG mouse Bmf
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17243||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAvailable from Addgene (#1767)
- Backbone size w/o insert (bp) 5330
Vector typeMammalian Expression, Retroviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt nameBcl2 modifying factor
SpeciesM. musculus (mouse)
Insert Size (bp)550
Entrez GeneBmf (a.k.a. AW260063, AW411872)
/ Fusion Protein
- 3xFLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
N-terminal FLAG epitopes were introduced through inclusion of corresponding sequence in the PCR primer.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe 3xFLAG mouse Bmf was a gift from Joan Brugge (Addgene plasmid # 17243 ; http://n2t.net/addgene:17243 ; RRID:Addgene_17243)
For your References section:Functional role and oncogene-regulated expression of the BH3-only factor Bmf in mammary epithelial anoikis and morphogenesis. Schmelzle T, Mailleux AA, Overholtzer M, Carroll JS, Solimini NL, Lightcap ES, Veiby OP, Brugge JS. Proc Natl Acad Sci U S A. 2007 Mar 6. 104(10):3787-92. 10.1073/pnas.0700115104 PubMed 17360431