R26-dCas9-VPR-mNG
(Plasmid #172441)

• Purpose: Rosa26 targeting vector, expresses dCas9-VPR and mNeonGreen
• Depositing Lab: Tyler Jacks
• Publication: Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-1360.e14. doi: 10.1016/j.ccell.2021.07.007. Epub 2021 Aug 5.

Backbone

• Vector backbone: R26 targeting vector
• Modifications to backbone: mutated sgRNA binding site in R26 homology arm
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: dCas9-VPR
• Insert Size (bp): 5796

Gene/Insert 2

• Gene/Insert name: mNeonGreen
• Insert Size (bp): 711

Resource Information

• Supplemental Documents:
  • R26-CAG-LSL-dCas9-VPR-P2A-mNG-WPRE-bGHpA.gbk
• A portion of this plasmid was derived from a plasmid made by: dCas9-VPR from Addgene 63798

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry