The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer.
Freed-Pastor WA, Lambert LJ, Ely ZA, Pattada NB, Bhutkar A, Eng G, Mercer KL, Garcia AP, Lin L, Rideout WM 3rd, Hwang WL, Schenkel JM, Jaeger AM, Bronson RT, Westcott PMK, Hether TD, Divakar P, Reeves JW, Deshpande V, Delorey T, Phillips D, Yilmaz OH, Regev A, Jacks T
Cancer Cell. 2021 Oct 11;39(10):1342-1360.e14. doi: 10.1016/j.ccell.2021.07.007. Epub 2021 Aug 5. PubMed Article
Freed-Pastor WA, Lambert LJ, Ely ZA, Pattada NB, Bhutkar A, Eng G, Mercer KL, Garcia AP, Lin L, Rideout WM 3rd, Hwang WL, Schenkel JM, Jaeger AM, Bronson RT, Westcott PMK, Hether TD, Divakar P, Reeves JW, Deshpande V, Delorey T, Phillips D, Yilmaz OH, Regev A, Jacks T
Cancer Cell. 2021 Oct 11;39(10):1342-1360.e14. doi: 10.1016/j.ccell.2021.07.007. Epub 2021 Aug 5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172433 | LV-PGK-Cre | Lentivirus, expresses Cre recombinase |
| 172434 | LV-PGK-Cre-EFS-mScarletSIIN | Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL |
| 172435 | LV-PGK-PVR-P2A-Cre-EFS-mScarletSIIN | Lentivirus, expresses Cre recombinase, mouse PVR (CD155), and mScarlet-SIINFEKL |
| 172436 | LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN | Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site |
| 172437 | U6-sgfiller-eCas9-T2A-BlastR | Expresses eCas9, BlastR and sgRNA cloning site |
| 172438 | H11-mScarletSIIN | Hipp11 targeting vector, expresses mScarlet-SIINFEKL |
| 172439 | H11-mScarletLAMA4 | Hipp11 targeting vector, expresses mScarlet-mLAMA4 |
| 172440 | H11-mScarletALG8 | Hipp11 targeting vector, expresses mScarlet-mALG8 |
| 172441 | R26-dCas9-VPR-mNG | Rosa26 targeting vector, expresses dCas9-VPR and mNeonGreen |
| 172492 | U6-sgH11-eCas9-T2A-BlastR | For CRISPR-assisted HDR, Hipp11 locus |
| 172493 | U6-sgR26-eCas9-T2A-BlastR | For CRISPR-assisted HDR, Rosa26 locus |