pCS2+-wbp2nl
(Plasmid #172463)

• Purpose: expresses wild-type protein when injected into embryos
• Depositing Lab: Sally Moody
• Publication: Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi: 10.1016/j.ydbio.2017.06.025. Epub 2017 Jun 27.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 5048
• Modifications to backbone: none
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: wbp2nl
• Species: X. laevis (frog)
• Insert Size (bp): 948
• Mutation: none
• Entrez Gene: wbp2nl.L (a.k.a. XELAEV_18023638mg, pawp, wbp2nl)
• Promoter: SP6
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Cla1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: M13

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Our group cloned it