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pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter
(Plasmid #172596)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172596 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 172596-D50 50 μg of DNA in Tris buffer $495
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DNA 172596-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10375
  • Vector type
    Mammalian Expression ; EGFP-PEST_Reporter
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myc-PDGFRA-R914W
  • Alt name
    Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
  • Alt name
    PDGFRA
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    3324
  • Mutation
    PDGFRA-R914W
  • Entrez Gene
    PDGFRA (a.k.a. CD140A, PDGFR-2, PDGFR2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Extracellular c-myc epitope tag (N terminal on insert)
    • Signal peptide from influenza HA (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Erik Procko (Addgene plasmid # 136456)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter was a gift from Daniel Kiss (Addgene plasmid # 172596 ; http://n2t.net/addgene:172596 ; RRID:Addgene_172596)

  • For your References section:

    Polygenic risk impacts PDGFRA mutation penetrance in non-syndromic cleft lip and palate. Yu Y, Alvarado R, Petty LE, Bohlender RJ, Shaw DM, Below JE, Bejar N, Ruiz OE, Tandon B, Eisenhoffer GT, Kiss DL, Huff CD, Letra A, Hecht JT. Hum Mol Genet. 2022 Jul 21;31(14):2348-2357. doi: 10.1093/hmg/ddac037. 10.1093/hmg/ddac037 PubMed 35147171
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