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Addgene

Spike Display_Part-4 DO
(Plasmid #172724)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172724 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO/intron
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 S HexaPro-D614G
  • Alt name
    SARS-CoV-2 S 6P-D614G
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site) replaced with 'GSAS'; F817P, A892P, A899P, A942P, K986P, V987P, D614G
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B TM (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Spike Display_Part-4 DO was a gift from IIya Finkelstein (Addgene plasmid # 172724 ; http://n2t.net/addgene:172724 ; RRID:Addgene_172724)

  • For your References section:

    Rapid characterization of spike variants via mammalian cell surface display. Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ. Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 10.1016/j.molcel.2021.11.024. 10.1016/j.molcel.2021.11.024 PubMed 34919820
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