pcDNA3.1(+)-C-6His-mEsrra
(Plasmid
#173152)
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Purposemouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells
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Depositing Lab
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Depositing OrganizationDuke-NUS Medical School
Located in Singapore -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173152 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 173152-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 173152-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1(+)-C-6His
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Backbone manufacturerGenescript
- Backbone size w/o insert (bp) 5432
- Total vector size (bp) 6710
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Modifications to backbonenone
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameORF clone Estrogen-related receptor alpha (Esrra)
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Alt nameERRa
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Alt nameEsrra
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SpeciesM. musculus (mouse)
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Insert Size (bp)1278
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MutationWild type
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GenBank IDNM_007953.2 NM_007953.2
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Entrez GeneEsrra (a.k.a. ERRalpha, Err1, Estrra, Nr3b1)
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer TAATACGACTCACTATAGG
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCustom generated by Genescript upon order.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.11.16.468891 for bioRxiv preprint.
DNA (Catalog # 173152-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 173152-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)-C-6His-mEsrra was a gift from Brijesh Kumar Singh (Addgene plasmid # 173152 ; http://n2t.net/addgene:173152 ; RRID:Addgene_173152) -
For your References section:
Esrra regulates Rplp1-mediated translation of lysosome proteins suppressed in metabolic dysfunction-associated steatohepatitis and reversed by alternate day fasting. Tripathi M, Gauthier K, Sandireddy R, Zhou J, Guptta P, Sakthivel S, Teo WW, Naing YT, Arul K, Tikno K, Park SH, Wu Y, Wang L, Bay BH, Sun L, Giguere V, Chow PKH, Ghosh S, McDonnell DP, Yen PM, Singh BK. Mol Metab. 2024 Sep;87:101997. doi: 10.1016/j.molmet.2024.101997. Epub 2024 Jul 19. 10.1016/j.molmet.2024.101997 PubMed 39032642
Map uploaded by the depositor.
