2XSmBiT-SARS-CoV-2 S1
(Plasmid
#173430)
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Purposeprotein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 Fc
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Depositing Lab
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Depositing OrganizationScripps California (The Scripps Research Institute)
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173430 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 173430-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 173430-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonephCMV3
- Backbone size w/o insert (bp) 4200
- Total vector size (bp) 7200
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name2XSmBiT SARS-CoV-2 S1-IgG1 Fc
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Alt namefusion protein of SmbiT, SARS-CoV-2 Spike S1 region and human IgG1 Fc
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SpeciesSARS-CoV-2
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Insert Size (bp)3000
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Mutationfurin cleavage site RRAR to GSAS
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GenBank IDMN908947
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
- Promoter CMV
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Tag
/ Fusion Protein
- human IgG1 Fc (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40 pA-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 173430-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 173430-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
2XSmBiT-SARS-CoV-2 S1 was a gift from David Nemazee (Addgene plasmid # 173430 ; http://n2t.net/addgene:173430 ; RRID:Addgene_173430) -
For your References section:
A Rapid Assay for SARS-CoV-2 Neutralizing Antibodies That Is Insensitive to Antiretroviral Drugs. Huang D, Tran JT, Peng L, Yang L, Suhandynata RT, Hoffman MA, Zhao F, Song G, He WT, Limbo O, Callaghan S, Landais E, Andrabi R, Sok D, Jardine JG, Burton DR, Voss JE, Fitzgerald RL, Nemazee D. J Immunol. 2021 Jul 1;207(1):344-351. doi: 10.4049/jimmunol.2100155. Epub 2021 Jun 28. 10.4049/jimmunol.2100155 PubMed 34183368
Map uploaded by the depositor.
