Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17355||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human), M. musculus (mouse)
Entrez GeneEIF4E2 (a.k.a. 4E-LP, 4EHP, EIF4EL3, IF4e, h4EHP)
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
pFLAG-4EHP-mISG15 expresses a FLAG tagged human 4EHP-mISG15(LRLRGG) fusion protein. STOP codon of 4EHP was removed by PCR. BamHI-mISG15-XbaI, which was made by PCR, was ligated to pFLAG-4EHP(stopless)/BamHI/XbaI sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFLAG-4EHP-mISG15 was a gift from Dong-Er Zhang (Addgene plasmid # 17355 ; http://n2t.net/addgene:17355 ; RRID:Addgene_17355)
For your References section:ISG15 modification of the eIF4E cognate 4EHP enhances cap structure-binding activity of 4EHP. Okumura F, Zou W, Zhang DE. Genes Dev. 2007 Feb 1. 21(3):255-60. 10.1101/gad.1521607 PubMed 17289916