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Addgene

pcDNA4/TO/GFP-GIGYF2
(Plasmid #141189)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141189 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA4/TO/GFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5078
  • Total vector size (bp) 9000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GIGYF2
  • Alt name
    GRB10-interacting GYF protein 2
  • Alt name
    PERQ amino acid-rich with GYF domain-containing protein 2
  • Alt name
    Trinucleotide repeat-containing gene 15 protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4000
  • Entrez Gene
    GIGYF2 (a.k.a. GYF2, PARK11, PERQ2, PERQ3, TNRC15)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4/TO/GFP-GIGYF2 was a gift from Simon Bekker-Jensen (Addgene plasmid # 141189 ; http://n2t.net/addgene:141189 ; RRID:Addgene_141189)
  • For your References section:

    GIGYF1/2-Driven Cooperation between ZNF598 and TTP in Posttranscriptional Regulation of Inflammatory Signaling. Tollenaere MAX, Tiedje C, Rasmussen S, Nielsen JC, Vind AC, Blasius M, Batth TS, Mailand N, Olsen JV, Gaestel M, Bekker-Jensen S. Cell Rep. 2019 Mar 26;26(13):3511-3521.e4. doi: 10.1016/j.celrep.2019.03.006. 10.1016/j.celrep.2019.03.006 PubMed 30917308