pIT5_KD_NahR
(Plasmid
#173778)
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Purpose(Empty Backbone) Bacterial genomic integration vector, NahR repressor
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173778 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepIT5
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EC100D pir+ from Epicentre
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pIT5_KD_NahR is designed to be integrated into the D145 attachment site, using the D145 integrase helper plasmid pHelper_D. This version of plasmid carries NahR repressor, and can be used for inducible gene expression (with sodium salicylate) following integration. The pIT5_KD_NahR need to be propagated in a pir+ strain of E. coli.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIT5_KD_NahR was a gift from Keith Shearwin (Addgene plasmid # 173778 ; http://n2t.net/addgene:173778 ; RRID:Addgene_173778) -
For your References section:
The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in E. coli. Hao N, Chen Q, Dodd IB, Shearwin KE. ACS Synth Biol. 2021 Jun 30. doi: 10.1021/acssynbio.1c00215. 10.1021/acssynbio.1c00215 PubMed 34190535
