Addgene: The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in E. coli.

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The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in E. coli.
Hao N, Chen Q, Dodd IB, Shearwin KE
ACS Synth Biol. 2021 Jun 30. doi: 10.1021/acssynbio.1c00215. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
173767	pIT5_CL	Bacterial genomic integration vector
173768	pIT5_TL	Bacterial genomic integration vector
173769	pIT5_SL	Bacterial genomic integration vector
173770	pIT5_KL	Bacterial genomic integration vector
173771	pIT5_KD	Bacterial genomic integration vector
173772	pIT5_KH	Bacterial genomic integration vector
173773	pIT5_KT	Bacterial genomic integration vector
173774	pIT5_KP	Bacterial genomic integration vector
173775	pIT5_KO	Bacterial genomic integration vector
173776	pIT5_KW	Bacterial genomic integration vector
173777	pIT5_KW_CymR	Bacterial genomic integration vector, CymR repressor
173778	pIT5_KD_NahR	Bacterial genomic integration vector, NahR repressor
173779	pIT5_KH_VanR	Bacterial genomic integration vector, VanR repressor
173780	pIT5_KP_LacI	Bacterial genomic integration vector, LacI repressor
173781	pHelper_D	The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.
173782	pAH69	The pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.
173783	pHelper_O	The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.
173784	pHelper_W	The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.
173785	pE_Cre_lambdaCos	The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.

Antibodies from Article