pSaPIPLC His
(Plasmid #173810)

• Purpose: Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells
• Depositing Labs: Anne Gershenson, Mary F Roberts
• Publication: Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87. doi: 10.1021/bi300057q. Epub 2012 Mar 16.

Backbone

• Vector backbone: pET-21a
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: E. coli BL21-Codonplus (DE3)-RIL can be used for expression. Note that this protein purification is performed at room temperature.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Staphylococcus aureus plc
• Alt name: Staphylococcus aureus 1-phosphatidylinositol phosphodiesterase
• Alt name: Staphylococcus aureus phosphatidyl-inositol specific phospholipase C
• Species: Other
• Mutation: none
• Promoter: T7
• Tag / Fusion Protein:
  • 6XHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See UniProt ID P45723 for the mature, untagged sequence.

Also see PubMed 30148347 (bacterial PI-PLC review).