PurposeExpression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||174081||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9015
- Total vector size (bp) 10350
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameNES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
Insert Size (bp)1335
MutationL270G, Y271L, N311G, C313A
- Promoter CMV
/ Fusion Protein
- NES-Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 was a gift from Ryan Mehl (Addgene plasmid # 174081 ; http://n2t.net/addgene:174081 ; RRID:Addgene_174081)
For your References section:Access to Faster Eukaryotic Cell Labeling with Encoded Tetrazine Amino Acids. Jang HS, Jana S, Blizzard RJ, Meeuwsen JC, Mehl RA. J Am Chem Soc. 2020 Apr 22;142(16):7245-7249. doi: 10.1021/jacs.9b11520. Epub 2020 Apr 10. 10.1021/jacs.9b11520 PubMed 32251579