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pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0
(Plasmid #174081)


Item Catalog # Description Quantity Price (USD)
Plasmid 174081 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.


  • Vector backbone
  • Backbone size w/o insert (bp) 9015
  • Total vector size (bp) 10350
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
  • Species
    Methanosarcina barkeri
  • Insert Size (bp)
  • Mutation
    L270G, Y271L, N311G, C313A
  • Promoter CMV
  • Tag / Fusion Protein
    • NES-Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 was a gift from Ryan Mehl (Addgene plasmid # 174081 ; ; RRID:Addgene_174081)
  • For your References section:

    Access to Faster Eukaryotic Cell Labeling with Encoded Tetrazine Amino Acids. Jang HS, Jana S, Blizzard RJ, Meeuwsen JC, Mehl RA. J Am Chem Soc. 2020 Apr 22;142(16):7245-7249. doi: 10.1021/jacs.9b11520. Epub 2020 Apr 10. 10.1021/jacs.9b11520 PubMed 32251579