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Access to Faster Eukaryotic Cell Labeling with Encoded Tetrazine Amino Acids.
Jang HS, Jana S, Blizzard RJ, Meeuwsen JC, Mehl RA
J Am Chem Soc. 2020 Apr 22;142(16):7245-7249. doi: 10.1021/jacs.9b11520. Epub 2020 Apr 10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
174080pDule-Tet3.0Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
174081pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

Antibodies from Article