PurposeTetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||174080||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3801
- Total vector size (bp) 5061
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Tetracycline, 10 μg/mL
Copy numberLow Copy
Gene/Insert nameTet3.0 tRNA synthetase and tRNA
Insert Size (bp)1260
MutationL270G, Y271L, N311G, C313A
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDule-Tet3.0 was a gift from Ryan Mehl (Addgene plasmid # 174080 ; http://n2t.net/addgene:174080 ; RRID:Addgene_174080)
For your References section:Access to Faster Eukaryotic Cell Labeling with Encoded Tetrazine Amino Acids. Jang HS, Jana S, Blizzard RJ, Meeuwsen JC, Mehl RA. J Am Chem Soc. 2020 Apr 22;142(16):7245-7249. doi: 10.1021/jacs.9b11520. Epub 2020 Apr 10. 10.1021/jacs.9b11520 PubMed 32251579