pEVOL-pylT-AznLRS
(Plasmid
#172482)
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PurposeFor E.Coli expression, 306L309L348I384F411W
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172482 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEVOL
- Total vector size (bp) 5651
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAznLRS and pylTcua
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SpeciesMethanosarcina mazei
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Insert Size (bp)1365
- Promoter araBAD
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer ATG CCA TAG CAT TTT TAT CC
- 3′ sequencing primer GAT TTA ATC TGT ATC AGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEVOL-pylT-AznLRS was a gift from Wenshe Liu (Addgene plasmid # 172482 ; http://n2t.net/addgene:172482 ; RRID:Addgene_172482) -
For your References section:
A Versatile Approach for Site-Specific Lysine Acylation in Proteins. Wang ZA, Kurra Y, Wang X, Zeng Y, Lee YJ, Sharma V, Lin H, Dai SY, Liu WR. Angew Chem Int Ed Engl. 2017 Feb 1;56(6):1643-1647. doi: 10.1002/anie.201611415. Epub 2017 Jan 2. 10.1002/anie.201611415 PubMed 28042700
