|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17410||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerJacks Lab
- Backbone size w/o insert (bp) 9200
Vector typeMammalian Expression, Lentiviral, Cre/Lox
Growth in Bacteria
Gene/Insert nameLet-7g mutant
Alt nameLet7 g
SpeciesM. musculus (mouse)
Insert Size (bp)500
Mutationmutated version of let-7g
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer n/a
- 3′ sequencing primer MSCV rev (Common Sequencing Primers)
Puro.let7gsm.Cre was generated by two rounds site-directed mutagenesis of Puro.let7g.Cre with mut1F (5’-GATTCCAGGCTGACCTAGTAGTTTGTACAGTTTGAGGGTCTATGATAC-3’), mut1R (5’-GTATCATAGACCCTCAAACTGTACAAACTACTAGGTCAGCCTGGAATC-3’), mut2F (5’-CAGGAGATAACTGTACAGGCCACTGGGTTGCCAGGAAC-3’), and mut2R (5’-GTTCCTGGCAACCCAGTGGCCTGTACAGTTATCTCCTG-3’).
For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Puro.let-7gsm.Cre was a gift from Tyler Jacks (Addgene plasmid # 17410 ; http://n2t.net/addgene:17410 ; RRID:Addgene_17410)
For your References section:Suppression of non-small cell lung tumor development by the let-7 microRNA family. Kumar MS, Erkeland SJ, Pester RE, Chen CY, Ebert MS, Sharp PA, Jacks T. Proc Natl Acad Sci U S A. 2008 Feb 28. ():. 10.1073/pnas.0712321105 PubMed 18308936